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SARS-CoV-2 RNA Polymerase inhibited by Remdesivir

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The outbreak of a novel coronavirus (SARS-CoV-2) represents a pandemic threat that has been declared a public health emergency of international concern. The SARS-CoV-2 RNA Polymerase is a key target for antiviral therapy. The 3D printed structure of SARS-CoV-2 RNA Polymerase inhibited by Remdesivir should enable the rapid development and evaluation of medical countermeasures to address the ongoing public health crisis.

Protein Description 7BV2

The replication of SARS-CoV-2 requires the viral RNA-dependent RNA polymerase (RdRp tinted Blue), a target of the antiviral drug, Remdesivir (Colored by Atom type). The complex structure reveals that the partial double-stranded RNA template (Red) is inserted into the central channel of the RdRp where Remdesivir is covalently incorporated into the primer strand (Magenta) at the first replicated base pair and terminates chain elongation.

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Here we report the cryo-EM structure of the SARS-CoV-2 RdRp either in the apo form at 2.8 Å resolution or in complex with a 50-base template-primer RNA and Remdesivir at 2.5 Å resolution. The complex structure reveals that the partial double-stranded RNA template is inserted into the central channel of the RdRp where Remdesivir is covalently incorporated into the primer strand at the first replicated base pair and terminates chain elongation. Our structures provide critical insights into the mechanism of viral RNA replication and a rational template for drug design to combat the viral infection.

Model Description

Biologic Model of SARS-CoV-2 RNA Polymerase inhibited by Remdesivir 3D printed in full-color sandstone and created from PDB ID: 7BV2