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RsmE-CsrA bound to mRNA

RsmE-CsrA bound to mRNA

This is a 3D print of RsmE bound to two strands of mRNA visualized as a ribbon bound to nucleotide sequences.

Protein Description 2JPP

Proteins of the RsmA/CsrA family are global translational regulators in many bacterial species. We have determined the solution structure of a complex formed between the RsmE protein, a member of this family from Pseudomonas fluorescens, and a target RNA encompassing the ribosome-binding site of the hcnA gene. The RsmE homodimer with its two RNA-binding sites makes optimal contact with an 5′-A/UCANGGANGU/A-3′ sequence in the mRNA. When tightly gripped by RsmE, the ANGGAN core folds into a loop, favoring the formation of a 3-base-pair stem by flanking nucleotides. We validated these findings by in vivo and in vitro mutational analyses. The structure of the complex explains well how, by sequestering the Shine-Dalgarno sequence, the RsmA/CsrA proteins repress translation.

Model Description

The RsmE homodimer is colored blue and purple according to protein’s beta sheet and helices. In red and orange are the two RNA-binding sites. mRNA is colored shades of green according to nucleotide type.

3D Print RsmE Bound to mRNA

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