RSV, Fusion, Glycoprotein, Prefusion, 5TDL

This is a 3D print of the RSV Fusion Glycoprotein in its prefusion state. The 3D printed protein models of RSV Fusion F Glycoprotein visualize the conformational changes of this viral protein during membrane fusion. Two chains are colored shades of magenta, with the third chain colored blue to red from N to C-Terminal.

Protein Description 5TDL

Bovine respiratory syncytial virus, a major cause of respiratory disease in calves, is closely related to human RSV, a leading cause of respiratory disease in infants. Recently, promising human RSV-vaccine candidates have been engineered that stabilize the metastable fusion (F) glycoprotein in its prefusion state; however, the absence of a relevant animal model for human RSV has complicated assessment of these vaccine candidates. Here, we use a combination of structure-based design, antigenic characterization, and X-ray crystallography to translate human RSV F stabilization into the bovine context. A “DS2” version of bovine respiratory syncytial virus F with subunits covalently fused, fusion peptide removed, and pre-fusion conformation stabilized by cavity-filling mutations and intra- and inter-protomer disulfides was recognized by pre-fusion-specific antibodies, AM14, D25, and MPE8, and elicited bovine respiratory syncytial virus-neutralizing titers in calves >100-fold higher than those elicited by post-fusion F. When challenged with a heterologous bovine respiratory syncytial virus, virus was not detected in nasal secretions nor in respiratory tract samples of DS2-immunized calves; by contrast bovine respiratory syncytial virus was detected in all post-fusion- and placebo-immunized calves. Our results demonstrate proof-of-concept that DS2-stabilized RSV F immunogens can induce highly protective immunity from RSV in a native host with implications for the efficacy of prefusion-stabilized F vaccines in humans and for the prevention of bovine respiratory syncytial virus in calves.

Fusion F, Glycoprotein, Prefusion, 5tdl, RSV, Respiratory Syncytial Virus
Fusion F, Glycoprotein, Postfusion, 5tdl, RSV, Respiratory Syncytial Virus
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RSV Fusion Glycoprotein Realtime-3D Biologic Explorer

Use the interactive realtime 3D Biologic Explorer to visualize the internal anatomy and dynamic shape changes of the RSV Fusion Glycoprotein. (Best viewed in a full-screen desktop browser).

[unity3d_game game=”FusionF_Explorer” name=”Fusion F Biologic Explorer” fullscreen=1 autorun=0 logo=1 spinner=1]

Model Description

3D printed protein models of RSV Fusion F Glycoprotein visualize the conformational changes of this viral protein during membrane fusion. Each chain is colored uniquely, with one chain colored blue to red from N to C-Terminal.

3D Print RSV Fusion F Glycoprotein-5TDL

Using the interface below, configure the scale and materials using the horizontal slider and drop-down menu.