This is a 3D print of the RSV Fusion Glycoprotein in its prefusion state. The 3D printed protein models of RSV Fusion F Glycoprotein visualize the conformational changes of this viral protein during membrane fusion. Two chains are colored shades of magenta, with the third chain colored blue to red from N to C-Terminal.
Protein Description 5TDL
Bovine respiratory syncytial virus, a major cause of respiratory disease in calves, is closely related to human RSV, a leading cause of respiratory disease in infants. Recently, promising human RSV-vaccine candidates have been engineered that stabilize the metastable fusion (F) glycoprotein in its prefusion state; however, the absence of a relevant animal model for human RSV has complicated assessment of these vaccine candidates. Here, we use a combination of structure-based design, antigenic characterization, and X-ray crystallography to translate human RSV F stabilization into the bovine context. A “DS2” version of bovine respiratory syncytial virus F with subunits covalently fused, fusion peptide removed, and pre-fusion conformation stabilized by cavity-filling mutations and intra- and inter-protomer disulfides was recognized by pre-fusion-specific antibodies, AM14, D25, and MPE8, and elicited bovine respiratory syncytial virus-neutralizing titers in calves >100-fold higher than those elicited by post-fusion F. When challenged with a heterologous bovine respiratory syncytial virus, virus was not detected in nasal secretions nor in respiratory tract samples of DS2-immunized calves; by contrast bovine respiratory syncytial virus was detected in all post-fusion- and placebo-immunized calves. Our results demonstrate proof-of-concept that DS2-stabilized RSV F immunogens can induce highly protective immunity from RSV in a native host with implications for the efficacy of prefusion-stabilized F vaccines in humans and for the prevention of bovine respiratory syncytial virus in calves.
RSV Fusion Glycoprotein Realtime-3D Biologic Explorer
Use the interactive realtime 3D Biologic Explorer to visualize the internal anatomy and dynamic shape changes of the RSV Fusion Glycoprotein. (Best viewed in a full-screen desktop browser).
[unity3d_game game=”FusionF_Explorer” name=”Fusion F Biologic Explorer” fullscreen=1 autorun=0 logo=1 spinner=1]
3D printed protein models of RSV Fusion F Glycoprotein visualize the conformational changes of this viral protein during membrane fusion. Each chain is colored uniquely, with one chain colored blue to red from N to C-Terminal.
3D Print RSV Fusion F Glycoprotein-5TDL
Using the interface below, configure the scale and materials using the horizontal slider and drop-down menu.
RSV Fusion Glycoprotein Base$28.00