CRISPR Cas9 bound to Guide RNA and Target DNA

CRISPR Cas9 printed in full-color sandstone colored purple according to the protein’s bFactor, it’s propensity for movement. RNA is colored red, DNA colored Yellow and Green.

Protein Description

“The RNA-guided DNA endonuclease CRISPR Cas9 cleaves double-stranded DNA targets with a protospacer adjacent motif (PAM) and complementarity to the guide RNA. Recently, we harnessed Staphylococcus aureus Cas9 (SaCas9), which is significantly smaller than Streptococcus pyogenes Cas9 (SpCas9), to facilitate efficient in vivo genome editing.


Here, we report the crystal structures of SaCas9 in complex with a single guide RNA (sgRNA) and its double-stranded DNA targets, containing the 5′-TTGAAT-3′ PAM and the 5′-TTGGGT-3′ PAM, at 2.6 and 2.7 Å resolutions, respectively. The structures revealed the mechanism of the relaxed recognition of the 5′-NNGRRT-3′ PAM by SaCas9. A structural comparison of SaCas9 with SpCas9 highlighted both structural conservation and divergence, explaining their distinct PAM specificities and orthologous sgRNA recognition. Finally, we applied the structural information about this minimal Cas9 to rationally design compact transcriptional activators and inducible nucleases, to further expand the CRISPR Cas9 genome editing toolbox.”
https://www.ncbi.nlm.nih.gov/pubmed/26317473

Purchase 3D Prints

You can purchase a 3D print of CRISPR-Cas9 on our shapeways.com website. The model can be printed in multiple sizes. Below is a preview of it printed in full-color sandstone. The magenta portions of the model are Cas9, red is the guide RNA, and the two strands of DNA are green and yellow.


Models available in multiple sizes.

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